NHE1 (Na, H Exchanger Isoform)

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue- specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.\n\nApplications: \nSuitable for use in Western Blot. Works poorly in Immunohistochemistry and Immunocytochemistry. Not suitable for use in Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:500 detected NHE1 on 10ug of human kidney lysates. Human kidney lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with N2015-02 (1:500). Proteins were visualized using an IgG (HRP) Gt xMo* and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\n*Recommended Secondary Antibodies:\n I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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