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NHE3 (Na, H Exchanger Isoform)

Cat no: N2017-22

NHE3 (Na, H Exchanger Isoform)

Na+/H+ exchangers are composed of a hydrophilic carboxy terminal domain and a hydrophobic, membrane spanning amino terminal domain. Functional studies indicate that it is the amino terminal domain which is responsible for the transmembrane exchange of sodium and hydrogen ions and that this activity is regulated by the interaction of cytosolic proteins with the hydrophilic carboxy terminal region.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: Detects a band at ~80kD in rabbit and ~83kD in rat.\nImmunoprecipitation: Use non-ionic detergent.\nImmunohistochemistry (rat): Detects NHE3 on PLP or parafomaldehyde fixed paraffin sections.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2017-22

Size

100ug

Applications

ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Rat, Rabbit

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Biemesderfer, et al., Am. J. Physiol. (1997) 273:F289-F299. 2. Biemesderfer, et al., J. Biol. Chem. (May 1998).

Additional Info

Recognizes the rabbit Na+/H+ Exchanger, Isoform NHE3. Species Crossreactivity: rat

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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