NHE6 (Na, H Exchanger 6)

Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular.\nA mammalian homolog of yeast NHA2 has been identified and classified as NHE6 (KIAA0267). NHE6 is 669 aa (mol wt ~75 kD), and predicted to contain 12 TM domains within the cytoplasmic N-terminus, the C-terminus may reside in the matrix. NHE6 is ubiquitously expressed but it is most abundant in mitochondrial-rich tissues such as brain, skeletal muscle, and heart.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:100,000 using 50-100ng control peptide/well. \nWestern Blot: 1-10ug/ml using Chemiluminescence. NHE6 has a calculated mol wt of ~75kD.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nN2020-60: NHE6, Human (Na, H Exchanger 6, KIAA0267)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2020-55

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Smith DR. 2004, Experimental Cell Research, Vol 298, Issue 2, 15 Aug 2004, Pgs 521-534. 2. Numata M et al (2001) JBC 276, 17386-17394; 3. Chris Yun CH et al (1995) Am J Physiol. 269, G1-G11 (Review); 4. Josette N and Pouyssegur J (1995) Am J Physiol. 268, C283-C296 (review).

Additional Info

Recognizes human NHE6. No significant homology exists with other NHE isoforms (NHE1-5).