NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)

Na+/H+ exchanger regulatory factor (NHERF1or EBP-50) is a member of the PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains and with the ERM family of proteins including merlin via its carboxy-terminus (2,3). NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1 and PDGFR. Thereby NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of the PI3K/Akt signaling in normal cells, and this mechanism may be disrupted in cancer (4). NHRF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in Cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting and trafficking (6). \n\nApplications: \nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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