Nibrin, p95, phosphorylated (Ser343) (ATV, AT-V1, AT-V2, Cell Cycle Regulatory Protein p95, FLJ10155, MGC87362, NBN, Nijmegen Breakage Syndrome, NBS, Nijmegen Breakage Syndrome Protein 1, NBS1, p95 NBS1)

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1:500-1:1000\nimmunohistochemistry: Paraffin embedded human tonsil tissue\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2498-03B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tentner AR, Lee MJ, Ostheimer GJ, et al. Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress. Mol. Syst. Biol. 20128:568. 2. Furusawa Y, Fujiwara Y, Campbell P, et al. DNA double-strand breaks induced by cavitational mechanical effects of ultrasound in cancer cell lines. PLoS ONE. 20127(1):e29012

Additional Info

Recognizes human Nibrin, p95 when phosphorylated at Ser343. Species Crossreactivity: rat.