Nickel BioAssay(TM) Kit

Nickel is one of four ferromagnetic elements (symbol Ni, at. Num. 28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. The Nickel Assay kit provides a simple method of quantifying Ni+2 in a variety of samples. The assay takes advantage of reaction of Ni+2 with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600nm. Fe+2 and Co+2 interfere with the assay, therefore extra steps (as described in protocol) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn+2, Cu+2, Zn+2) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni+2 in a variety of samples, which gives a linear range of 2 to 50nmol Nickel containing less than 25nmol Cobalt.\n\nKit Components:\nNickel Assay Buffer 1x20ml\nNickel Reagent 1x1ml\nNickel Chloride Standard (1.0umol) lyophilized.\n\nStorage and Stability:\nStore the kit at RT. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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