Niemann-Pick Disease, Type C1 Gene-Like 1 (NPC1L1, Niemann-Pick C1-like Protein 1, NPC1 like 1, NPC1 Niemann-Pick Disease Type C1 Gene-like 1, NPC1L1 Splice Variant, Truncated Niemann-Pick C1-like Protein 1)

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2566-32C

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

As reported

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Altmann, S.W., et al. Niemann-Pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science. 303:1201-1204, 2004. \n2. Klett, E.L., & Patel, S.B. Will the real cholesterol transporter please stand up. Science. 303:1149-1150, 2004. \n3. Frolov, A., et al. NPC1 and NPC2 regulate cellular cholesterol homeostasis through generation of low density lipoprotein cholesterol-derive oxysterols. J. Biol. Chem. 278(28): 25517-25525, 2003. \n4. Somers, K.L., et al. Mutation analysis of feline Niemann-Pick C1 disease. Mol. Genet. Metab. 79(2): 99-103, 2003. \n5. Differential trafficking of the Niemann-Pick C1 and 2 proteins highlights distinct roles in late endocyte lipid trafficking. Acta. Pediatr. Suppl. 92(443): 63-73, 2003. \n6. Reid, P.C., et al. Trafficking defects in endogenously synthesized cholesterol in fibroblasts, macrophages, hepatocytes, and glial cells from Niemann-Pick type C1 mice. J. Lipid Res. 44(5): 1010-1019, 2003. \n7. Altmann, S.W., et al. Niemann-Pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science. 303(5661): 1149-1150, 2004.\n8. Iannou, Y.A., et al. The sructure and function of the Niemann-Pick C1 protein. Mol. Genet. Metab. 71(1-2): 175-181, 2000.\n9. Identification of 58 novel mutations in Niemann-Pick disease type C: correlation with biochemical phenotype and importance of PTC1-like domains in NPC1. Hum Metab. 22(4): 313-325, 2003.

Additional Info

This antibody is specific for NPC1L1 protein. Species Crossreactivity: This antibody reacts with rat and human NPC1L1 protein. There is 94% homology for mouse.