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NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1, Activator of RNA Decay, ARD1, PPP1R8, Protein Phosphatase 1 Regulatory Subunit 8)

Cat no: N2568

NIPP1 (Nuclear Inhibitor of Protein Phosphatase 1, Activator of RNA Decay, ARD1, PPP1R8, Protein Phosphatase 1 Regulatory Subunit 8)

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml. Detects a band of ~40kD (predicted molecular weight: 40kD) in human lung and H460 cell extracts. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2568

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse

Form

Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human NIPP1. Expected to crossreact with Mouse (100% identity with immunogen) due to sequence homology.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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