NIPSNAP3A (Protein NipSnap Homolog 3A, NipSnap3A, Protein NipSnap Homolog 4, NipSnap4, Target for Salmonella Secreted Protein C, TassC, NIPSNAP4, HSPC299)

The NIPSNAP proteins comprise a family of evolutionarily well-conserved proteins with strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. NIPSNAP2, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumors. While NIPSNAP3B is highly expressed skeletal muscle, NIPSNAP3A mRNA levels are low. NIPSNAP3A protein is associated with plasma membrane and partially localized in rafts. NIPSNAP proteins have been suggested to be important in vesicular transport. At least two isoforms of NIPSNAP3A are known to exist. NIPSNAP3A antibody is predicted to not cross-react with any other members of the NIPSNAP protein family.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse Brain Tissue Lysate \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

146354

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Seroussi E, Pan HQ, Kedra D, et al. Characterization of the human NIPSNAP1 gene from 22q12: a member of a novel gene family. Gene 1998; 212:13-20. 2. Wang X-Y, Smith DI, Liu W, et al. GBAS, a novel gene encoding a protein with tyrosine phosphorylation sites and a transmembrane domain, is co-amplified with EGFR. Genomics 1998; 49:448-51. 3. Buechler C, Bodzioch M, Bared SM, et al. Expression pattern and raft association of NIPSNAP3 and NIPSNAP4, highly homologous proteins encoded by genes in close proximity to the ATP-binding cassette transporter A1. Genomics 2004; 83:1116-24.

Additional Info

Recognizes human NIPSNAP3A. Species Crossreactivity: mouse.