Nitrated MW Standards, Western Blot (SOD, SOD Dimer, BSA, Myosin)

Western Blot nitrated MW Standards for N2700-02 and N2700-13.\n\nNitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.\n\nApplications:\nSuitable for use in Western Blot using the polyclonal anti-nitrotyrosine. Not sutiable for use with monoclonal anti-nitrotyrosine.\n\nRecommended Dilutions:\nWestern Blot: 10ul per lane.\nOptimal dilutions to be determined by researcher.\n\n\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2700-10

Size

50ul

Applications

WB

Form

Supplied in SDS-PAGE sample buffer, reduced.

Purity

Highly Purified

References

1. Viera, L., Methods Enzymol. 301: 373-381 (1999). 2. Beckman, et al., Nature 364: 584 (1993). 3. Haddad, et al., J. Clin. Invest. 94: 2407 (1994). 4. Ayata, et al., J. Neurosci. 17: 6908 (1997).

Additional Info

A mixture of molecular weight calibrated proteins that were nitrated with peroxynitrite: Superoxide dismutase: ~16kD; Superoxide dismutase (dimer): ~32kD; BSA: ~66kD; Myosin, muscle: ~215kD).