Nitric Oxide Assay Kit, BioAssay(TM)

Description:\nNitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins and direct covalent interaction with functional biomolecules.\nSimple, direct and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure for NO level. Nitric Oxide Assay Kit is designed to accurately measure NO production following reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to only 40 min.\n\nKey Features:\nSensitive and accurate. Detection range 0.1 - 50uM in 96-well plate.\nRapid and reliable. Using optimized Cd/Cu reagent, the time required for reduction of NO3- to NO2- is 15 min at > 98.5% conversion rate.\nSimple and high-throughput. The procedure involves mixing sample with two reagents, incubation for 5 min and reading the optical density. Can be readily automated to measure thousands of samples per day.\n\nApplications:\nDirect Assays: NO in plasma, serum, urine, tissue/cells and foods.\nDrug Discovery/Pharmacology: effects of drugs on NO metabolism.\n\nKit Contents (250 tests in 96-well plates):\nReagent A:\n14ml\nReagent B:\n14ml\n20X ZnSO4:\n1.0ml 1.5M solution\n30X NaOH:\n1.0ml 1.65M solution\n1X Glycine Buffer:\n30ml\n3X Activation Buffer:\n50ml\nCadmium granules:\n15g\nNitrite standard:\n1.0ml 1.0mM nitrite\nStorage conditions. Store Reagent A, B and Nitrite standard at 4 degrees C. All other components can be stored at room temp. Shelf life: 12 months.\nPrecautions: reagents are for research use only. Please refer to Material Safety Data Sheet for detailed information.

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SPECIFICATIONS

Catalog Number

N2576-98

Size

250Tests

References

1. Cortas, N.K. & Wakid, N.W., Clin. Chem. 36: 1440-1443 (1990). 2. Ridnour, L.A., et al., Anal. Biochem. 281: 223-229 (2000). 3. Sen, N.P. & Donaldson, B., J. Assoc. Off. Anal. Chem. 61: 1389-1394 (1978).