Nitric Oxide (NO) Enzymatic Assay Kit, BioAssay(TM)

The Analyte:\nThis kit employs immunoaffinity purified Nitrate Reductase to allow you to measure total nitric oxide (NO) produced in in vitro experimental systems following enzymatic conversion to nitrite. \n\nNitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase (NaR), followed by quantitation of nitrite using Griess Reagent. \n\nIn addition to providing all necessary components in a microtiter format, this kit employs affinity purified nitrate reductase and NADH, thereby circumventing some of the potential problems reported for nitric oxide measurement using NADPH dependent nitrate reductases.\n\nPrinciple of the Assay:\nIn aqueous solution, nitric oxide rapidly degrades to nitrate and nitrite. Spectrophotometric quantitation of nitrite using Griess Reagent is straightforward, but does not measure nitrate. This kit employs the NADH-dependent enzyme nitrate reductase (NaR) for quantitative conversion of nitrate to nitrite prior to quantitation of nitrate using Griess reagent

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