Nitric Oxide Synthase, Brain (1-181) NOS1 (NOS, bNOS, nNOS, IHPS1, N-NOS, NC-NOS, Neuronal NOS, Peptidyl-cysteine S-nitrosylase NOS1)

Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is thought to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal (Type I) nitric oxide synthase exhibit relatively normal brain development and behavior. \n\nUniProt Number:\nHuman(P29475), Mouse(Q9Z0J4), Rat(P29476)\n\nGene ID:\nNOS1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144968

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Rat, Goat, Prc

Form

Ascites, supplied as a lyophilized powder. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

P Type

Mab

Isotype

IgG1

References

1. Brenman, J. E.; Chao, D. S.; Xia, H.; Aldape, K.; Bredt, D. S. : Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy. Cell 82: 743-752, 1995.\n2. Wang, Y.; Goligorsky, M. S.; Lin, M.; Wilcox, J. N.; Marsden, P. A. : A novel, testis-specific mRNA transcript encoding an NH(2)-terminal truncated nitric-oxide synthase. J. Biol. Chem. 272: 11392-11401, 1997.\n3. Xie, J.; Roddy, P.; Rife, T. K.; Murad, F.; Young, A. P. : Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription. Proc. Nat. Acad. Sci. 92: 1242-1246, 1995.\n4. Kharazia, V. N.; Schmidt, H. H. H. W.; Weinberg, R. J. : Type I nitric oxide synthase fully accounts for NADPH-diaphorase in rat striatum, but not cortex. Neuroscience 62: 983-987, 1994.

Additional Info

Recognizes human, rat, goat and porcine Nitric Oxide Synthase, Brain (1-181), alpha. No cross reactivity with other proteins.