Nitrotyrosine Immunoblotting Control (nTyr)

A combination of three proteins: nitrated bovine superoxide dismutase (SOD) ~16kD, nitrated bovine serum albumin (BSA) ~66kD, and nitrated rabbit muscle myosin ~215kD. A dimer of SOD is occasionally observed at ~32kD. The proteins were nitrated using peroxynitrite.\n\nProtein Concentration: Nitrated-myosin ~10ug/ml; nitrated-BSA ~50ug/ml; nitrated-SOD ~10ug/ml. Total protein concentration: ~70ug/ml. \n\nApplications:\nSuitable for Western Blot.\n\nRecommended Dilution:\nDilute 1:1 with Reducing Sample Buffer and boil for five minutes to reduce the preparation. Load 20ul of reduced control per lane for immunoblot analysis. This preparation may be used as a positive control for N2700-05 Mab and N2700-06 Pab Nitrotyrosine (nTyr) antibodies. \n\nStorage and Stability: \nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2705

Size

40 Blots

Applications

WB

Form

Supplied as a liquid in PBS, pH 7.2.

References

Beckman, J.S., et al., Biol. Chem. Hoppe-Seyler 375: 81, 1994 Beckman, J.S., et al., Nature 364: 584, 1993 Ischiropoulos, H., et al., Arch. Biochem. Biophys. 298: 431, 1992 Kono, S., et al., Biochem Biophys. Res. Comm. 190: 283, 1993 Ohshima, H., et al., Ed. Chem. Tox. 28: 647, 1990. Ye, Y.Z., et al., Meth Enzymol. 269: 201, 1996