Nitrotyrosine (nTyr)

Nitric oxide (NO) is implicated in carcinogenesis(1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in these tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry (ABC detection method). Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunocytochemistry (ABC): 1:50\nELISA: 1:1000\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2700-01

Size

100ul

Applications

ELISA, ICC, WB

Hosts

Rabbit

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Bentz, B.G. et al. (2000) Head Neck 22, 64-70. 2. Jaiswal, M. et al. (2000) Cancer Res. 60, 184-190. 3. Olivenza, R. et al. (2000) J. Neurochem. 74, 4. MacMillan-Crow, L.A. et al. (1996) Proc. Natl.Acad. Sci. USA 93, 11853-11858. 5. Chazotte-Aubert, L. et al. (2000) Biochem. Biophys. Res. Commun. 267, 609-613. 6. Sorensen, L. et al. (2001) J. Neurosci. 21(20), 8082-8090.

Additional Info

Detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. Does not crossreact with unmodified tyrosine or with phospho-tyrosine.