Nitrotyrosine (nTyr)

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. \nNitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitrotyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).\n\nApplications:\nSuitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 0.7ug/ml detects 5ug SIN-1 treated BSA using Goat anti-mouse IgG-HRP as the secondary antibody.\nOptimal dilutions to be determined by the researcher. \n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2700-03B

Size

25ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Girault, I., et al., Free Radical Biology and Medicine 31(11): 1375-1387 (2001). 2. Gow, A.J., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 287(2): L262-L268 (2004). 3. Takemoto, K., et al., Acta Med. Okayama 61(1): 17-30 (2007). 4. Reynolds, M.R., et al., J. Nerosci. 26(42): 10,636-10,645 (2006). 5. Pfister, H., et al., Vet. Pathol. 39: 190-199 (2002). 6. Khan, J., et al., Biochem J. 330(2): 795-801 (1998).

Additional Info

Recognizes 3-nitrotyrosine moieties. No detectable crossreactivity with non-nitrated tyrosine. Not species specific. Species Crossreactivity: Human, mouse, rat and canine.