Nitrotyrosine (nTyr)

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins.\nImmunocytochemistry: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Controls:\nImmunohistochemistry: Human Alzheimer diease brain sections (Methacarn fixed), Mouse livers\nWestern Blot: Nitrated-BSA\nIndirect ELISA: Nitrated-BSA\nIndirect Immunofluorescence: A549 cells metabolically labeled with 3-nitrosine\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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