Nitrotyrosine (nTyr)

Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml. Proteins extracted from human A431 cells or other cells pretreated with peroxynitrite for 5 min may serve as good positive control.\nELISA: 1:10,000-1:50,000 using 50-100ng N2700-10 Nitrated MW Standards, Western Blot/well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2700-11

Size

50ug

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, BSA, 0.025% sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1

References

1. Viera, L., Methods Enzymol. 301: 373-381 (1999). 2. Beckman, et al., Nature 364: 584 (1993). 3. Haddad, et al., J. Clin. Invest. 94: 2407 (1994). 4. Ayata, et al., J. Neurosci. 17: 6908 (1997).

Additional Info

Recognizes nitrated proteins from all species.