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Nitrotyrosine (nTyr)

Cat no: N2700-16D

Nitrotyrosine (nTyr)

Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 2-100ng/ml\nWestern Blot: 0.1ug/ml\nImmunoprecipitation: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nCrossreactivity Data:\nSpecificity as determined by ELISA competition experiments.\n\nCompound Inhibition\nNitrotyrosine 95%\nNitrotryptophan 4%\nChlorotyrosine 3%\nPhosphotyrosine 2%\nTyrosine 0% \nAminotyrosine 0%\n\nRecommended Secondary Antibodies:\nI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo\nI1904-65N: IgG (HRP) Pab Rb xMo\nI1903-07E: IgG (HRP) Pab Rb xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2700-16D

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro hemoglobin.

Alternative Names

Tyr-NO2

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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