NK1.1 Cell Surface Antigen (Killer Cell Lectin-like Receptor Subfamily B Member 1C, CD161 Antigen-like Family Member C, Lymphocyte Antigen 55c, Ly-55c, NKR-P1.9, NKR-P1C, Natural Killer Cell Surface Protein P1-40, NKR-P1 40, CD161c, Klrb1c, Ly55c, Nkrp1c)

Clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161 and the mouse proteins have been referred to as CD161a, -b, -c etc. Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by 3H2788 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.\n\nThis antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells. \n\nFusion Partners: Spleen cells from immunized (C3HxBALB/c) FI Hybrid were fused with cells of the Sp2/0-Ag14 myeloma cell line.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation, and functional assays (in vivo depletion of NK cells, and in vitro activation of NK cells). Other applications have not been tested. \n\nRecommended Dilution:\nFlow Cytometry: 5-10ug/ml. Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul.\nFunctional Assays: 1-10ug/ml\nOptimal dilutions to be determined by researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\n\n \n

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SPECIFICATIONS

Catalog Number

N2851-75A

Size

1mg

Applications

FC, IP

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Koo, G.C. and Peppard, J.R. (1981) Hybridoma 3: 301

Additional Info

Recognizes the mouse NK1.1 cell surface antigen. Useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Species Crossreactivity: Does not crossreact with rat or human.