NKCC1 (Sodium, Potassium, Chloride Cotransporter 1, BSC2, CCC1)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+-K+-Cl- (NKCC) co- transporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1) and the K+Cl- cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500aa), and long hydrophilic, intracellular N- and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide. NKCC1/BSC2 (human 1212aa, rat 1203aa, and mouse 1205 aa; calculated mol. wt of ~115kD; actual size is ~145kD) is strongly expressed in the kidney and many other tissues. In mouse NKCC1 is detected in basolateral membrane of IMCD and in mesangial cells in the glomerulus and basolateral membrane of acinar cells in rat submandubular gland. In the brain, NKCC1 mRNA is most prominent in choroid plexus.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL). Full length human NKCC1 is ~170kD. \nELISA: 0.5-1.0ug/ml. Using 1ug/ml N2800A control peptide per well. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2800

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Moore-Hoon, M.L., et al., Biochim. Biophys. Acta 1373: 261 (1998). 2. Delpire, E., et al., J. Biol. Chem. 269: 25,677 (1994). 3. Payne, J.A., et al., J. Biol. Chem. 270: 17,977 (1995). 4. Isering, P., et al., J. Biol. Chem. 272: 24,556 (1997). 5. Mount, D.B., et al., J. Exptl. Biol. 201: 2091-2102 (1998) (review).

Additional Info

Recognizes rat sodium, potassium, chloride cotransporter 1 (NKCC1, BSC2, CCC1). No significant sequence homology with other CCC. Species Sequence Homology: mouse: 95%; human: 86%; bovine: 82%.