NKCC2 (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. It includes the thiazide-sensitive Na+Cl- cotransporters (NCC or TSC), the loop diuretics-sensitive Na+K+Cl- (NKCC) cotransporters (NKCC1/CCC1/BSC2 and NKCC2/CCC2/BSC1), and the K+Cl- cotransporters (KCC1-4). These co-transporters share a common predicted membrane topology, with 12 TM domains (~500 aa), and long hydrophilic, intracellular N-and C-termini containing regulatory phosphorylation sites. NKCC transport Na, K, and Cl ions into and out of a wide variety of epithelial and non-epithelial cells. The transport process is characterized by electroneutrality (almost always with stoichiometry of 1Na:1K:2Cl) and inhibition by the loop diuretic bumetanide, benzametanide, and furosemide.\n\nUnlike NKCC1, NKCC2 (human 1099 aa, rat/mouse 1095 aa; calculated mol size of ~121kD; actual size ~150kD; ~45% identity with NKCC1) is most strongly expressed in the kidney (inner and outer stripes of medulla and cortical thick ascending limb), and macula densa. NKCC2 plays a critical role in transcellular absorption of Na+Cl- by the medullary and cortical TALH, and a secondary role in the paracellular transport of Na-Ca and Mg. Mutations in the NKCC2 gene result in Bartter's syndrome, an inherited disease characterized by hypokalemic metabolic alkalosis, hypercalciuria, salt wasting, and volume depletion.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000. Full length huma NKCC2 is ~150kD. \nELISA: 1:10,000-1:50,000. Using 1ug/ml control peptide per well. \nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN2801A: NKCC2, Control Peptide (Sodium, Potassium, Chloride Cotransporter 2, BSC1, CCC2)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2801-01

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Gamba G et al (1994) J Biol. Chem. 269:17713; 2. Simon DB et al (1996) Nature Genet. 13:183; 3. Mount DB et al (1995) J Am. Soc. Nephrol. 6:347; 4. Igarashi P et al (1995) Am. J. Physiol. 269:F405; 5. Haas M et al (1987) Am. J. Physiol. 253:C243; 6. Mount DB et al (1998) J. Exptl. Biol. 201:2091 (review).\nCitations:\n1. Velic A, 2005, Am. J of Transplantation, 5 ,1276-1285, WB, IHC 2. Markossian S, 2005, Cytokine 30, 319-327, WB, 3. Kida Y, 2007, J. Histochem. Cytochem., in press, IHC. 4. Earnest J N, 2007, Brain Research, 1144, 231-238, WB, 5. Kreydiyyeh SI, 2004, Cytokine, 26, 1-8, WB, 6. Kreydiyyeh SI, 2006, Cytokine, 33, 138-144, WB, 7. Liu L, 2007, J. Am. Soc. Nephrol., Dec 2007; 18: 3067 - 3077, IHC 8. Quaglia NB, 2004, \"Clin. Exp. Pharmacol. Physiology 31, 4, 231-236\", WB, 9. Ernest NJ, 2007, Brain Research, 1144, 231-238, WB,

Additional Info

Recognizes rat NKCC2, BSC1 and CCC2. The immunogen shows no significant sequence homology with NKCC1 or TSC. Species sequence homology: mouse-100%, human and rabbit- 87%.