NKp46, Natural Cytotoxicity Receptor, Recombinant, Human (NCR NKp46, Natural Killer Cell Activating Receptor NKp46)

A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors(NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30,which are expressed on circulating NKcells, and NKp44,which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46( 22-255aa) purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.\n\nDescription: \nRecombinant Human NRNKp46 produced in E.Coli is a single,non-glycosylated polypeptide chain containing 235 amino acdis and having a molecular mass of 26.6kD.\n\nSequence: \nMQQQTLPKPF IWAEPHFMVP KEKQVTICCQ GNYGAVEYQL HFEGSLFAVD RPKPPERINKVKFYIPDMNS RMAGQYSCIY RVGELWSEPS NLLDLVVTEM YDTPTLSVHP GPEVISGEEV TFYCRLDTAT SMFLLLKEGR SSHVQRGYGK VQAEFPLGPV TTAHRGTYRX FGSYNNHAWSFPSEPVKLLV TGDIENTSLA PEDPTFSADT WGTYLLTTET GLQKDHALWD HTAQN\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2835A

Size

5ug

Form

Supplied as a liquid in PBS, pH 7.4 and 1mM EDTA.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status.\n\nBMC Immunol 2006 Apr 27;7:10\n\n2. NKp46 and NKG2D receptor expression in NK cells with CD56dim and CD56bright phenotype: regulation by histamine and reactive oxygen species.\n\nBr J Haematol 2006 Jan;132(1):91-8\n\n3. Characterization of the heparin/heparan sulfate binding site of the natural cytotoxicity receptor NKp46.\n\nBiochemistry 2005 Nov 8;44(44):14477-85\n\n4. Expression of natural cytotoxicity receptors NKp30, NKp44, and NKp46 mRNAs and proteins by human hematopoietic and non-hematopoietic cells.\n\nLeuk Res 2006 Jan;30(1):37-46\n\n5. Effects of prolactin and cortisol on natural killer (NK) cell surface expression and function of human natural cytotoxicity receptors (NKp46, NKp44 and NKp30).\n\nClin Exp Immunol 2005 Feb;139(2):287-96\n\n6. Rat NKp46 activates natural killer cell cytotoxicity and is associated with FcepsilonRIgamma and CD3zeta.\n\nJ Leukoc Biol 2004 Dec;76(6):1200-6