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NKX3.1 (Homeobox Protein Nkx-3.1, Homeobox Protein NK-3 Homolog A, NKX3-1, NKX3A)

Cat no: N2850-30P

NKX3.1 (Homeobox Protein Nkx-3.1, Homeobox Protein NK-3 Homolog A, NKX3-1, NKX3A)

NKX3.1, a 234aa tumor suppressor protein, is a novel member of NK Class of Homeodomain proteins. It is a human homolog of NK3 (Drosophila) and Nkx3.1 (Mouse) and consists of a highly conserved DNA-binding Homeodomain. It plays a vital role in prostrate epithelial cell development, differentiation and carcinogenesis. Its expression is androgen-regulated and has growth suppression and differentiation effects in prostrate epithelial cells. It acts as a transcriptional repressor of PSA promoter by interacting with Prostrate-derived Ets factor. Its role has also been implicated in growth of testicular epithelial cells. It is specifically expressed in prostrate and testis & loss of expression has been detected in prostrate carcinomas, prostatic intraepithelial neoplasia and testicular cancers of germ cell origin.\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:200-1:400\nWestern Blot: 1:500-1:2000\nImmunohistochemistry (Formalin fixed paraffin embedded): 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2850-30P

Size

50ul

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG2b

Additional Info

Recognizes human NKX3-1.

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