Nkx3.1 (NK-2 Family Homeodomain Transcription Factor)

The Nkx3.1 protein is the product of a prostate-specific regulatory gene known as the NKX3.1 homeobox gene. NKX3.1 maps to chromosome band 8p21. Loss of heterozygosity at this particular site has been associated with tissue dedifferentiation and loss of androgen responsiveness in the progression of prostate cancer. Loss of specific regions of chromosome 8 occur in 60-80% of prostate tumors. Although a broad range of tumor suppressor genes, including NKX3.1, PTEN, Rb, p27, p16, and Myc, have been proposed as the genetic loci directly responsible for the chromosomal abnormalities characteristic of prostate cancer, none of these genes has consistently demonstrated mutations in a large population of prostate cancer specimens. Mouse studies of NKX3.1 have demonstrated that the gene is essential for normal prostate morphogenesis and function. Inactivation produces prostatic epithelial hyperplasia and dysplasia, closely modeling a preneoplastic condition. In vitro, overexpression of human and mouse NKX3.1 suppresses growth and tumorigenicity of prostate carcinoma cells. These findings suggest that NKX3.1 may be responsible for maintaining the normal prostate, and its loss may represent a predisposing event for prostate carcinogenesis. \n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen and paraffin): 10-20ug/ml\nWestern Blot: 1-3ug/ml\nELISA: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2850-30B

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG1

References

1. He, W.W., et al. Genomics 43:69-77 (1997). 2. Chang, M., et al. Am J Pathol 144:1-6 (1994). 3. Matsuyama, H., et al. Oncogene 9:3071-3076 (1994). 4. Abate-Shen, C., and Shen, M.M..Genes & Devel 14:2410-2434 (2000). 5. Bhatia-Gaur, R., et al. Genes & Devel 13:966-977 (1999). 6. Kim, M.J., et al. PNAS 99:2884-2889 (2002).

Additional Info

Recognizes human Nkx3.1 protein at ~34kD. Does not crossreact with mouse. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.