NLRC4 (Nucleotide-binding Oligomerization Domain, NOD, (NOD)-like Receptor C4)

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NOD and carboxy-terminal leucine-rich-repeat (LRR) domain involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-kB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). The NLRC4 (IPAF) inflammasome forms in response to bacterial flagellin as well as components of the bacterial conserved type II secretion system (TTSS) (8-12). Ligand detection and ligand-dependent NLRC4 oligomerization and inflammasome activation require the NAIP family of proteins (13,14). In mice, NAIP5 and NAIP6 associate with flagellin, while NAIP2 interacts with TTSS rod proteins (13,14). In humans, NAIP recognizes the TTSS needle protein Cprl (14). In addition, NLRC4 is phosphorylated by PKCd in response to bacterial infection and this phosphorylation is required for inflammasome assembly and caspase-1 activation (15).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137475

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied in 10 mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol and <0.02% sodium azide.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Elinav, E. et al. (2011) Immunity 34, 665-79. 2. Inohara, N. et al. (1999) J Biol Chem 274, 14560-7. 3. Ogura, Y. et al. (2001) J Biol Chem 276, 4812-8. 4. Sabbah, A. et al. (2009) Nat Immunol 10, 1073-80. 5. Mariathasan, S. et al. (2004) Nature 430, 213-8. 6. Agostini, L. et al. (2004) Immunity 20, 319-25. 7. Martinon, F. et al. (2002) Mol Cell 10, 417-26. 8. Poyet, J.L. et al. (2001) J Biol Chem 276, 28309-13. 9. Mariathasan, S. et al. (2004) Nature 430, 213-8. 10. Franchi, L. et al. (2006) Nat Immunol 7, 576-82. 11. Miao, E.A. et al. (2006) Nat Immunol 7, 569-75. 12. Miao, E.A. et al. (2010) Proc Natl Acad Sci U S A 107, 3076-80. 13. Kofoed, E.M. and Vance, R.E. (2011) Nature 477, 592-5. 14. Zhao, Y. et al. (2011) Nature 477, 596-600. 15. Qu, Y. et al. (2012) Nature 490, 539-42.

Additional Info

Recognizes endogenous levels of total human NLRC4.