NMDA 1 Receptor, C1 Splice Variant (NMDA R1, N-methyl-D-aspartate)

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor is a heteromultimer of at least two NR1 (NMDA receptor-1) subunits and two NR2 subunits. Regulation of the NMDA complex by external factors such as zinc, PKC and polyamines is mediated by NR1 subunits. Upon glycine binding to NR1, and glutamate binding to NR2, the NMDA channel is opened, allowing calcium and sodium influx into the cell. NR1 is a 120 kD, 938 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 541 aa extracellular domain and a 105 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. Differential splicing of three exons generates up to nine isoforms of NR1. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Eight are integral membrane proteins. At the N-terminus, four isoforms show a 21 aa insertion between aa 190-191, and four do not. This is compounded by at least four possible variants between aa 864-938 in the cytoplasmic tail that impact potential serine phosphorylation sites. To date, all are believed to be physiologically active. There is one potentially "soluble" form that is only 181 aa in length. Its function is unknown. \n\nApplications:\nWestern Blot: 1:1000 \nImmunohistochemistry: 1:1000 to 1:2000 (frozen sections) \nOptimal dilutions should be determined by each laboratory for each application. \n \nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2904A

Size

25ug

Applications

IHC, WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder in 5mM ammonium bicarbonate.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233. \n2. Sugihara, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:826. \n3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673. \n4. Moriyoshi, K. et al. (1991) Nature 354:31.

Additional Info

Recognizes mouse and rat ~120kD NR1 subunit of the NMDA Receptor containing the C1 splice variant.