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NMDA 1 Receptor, phosphorylated (Ser896) (NMDA R1, N-methyl-D-aspartate)

Cat no: N2904-26A

NMDA 1 Receptor, phosphorylated (Ser896) (NMDA R1, N-methyl-D-aspartate)

N-methyl-D-aspartate receptor (NMDAR) mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment and neuroplasticity (1). The receptor function can be regulated by the protein kinase/phosphatase complex associated with it (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor on Ser890/ Ser896 and PKA can phosphorylate NR1 on serine 897 (3). The phosphorylation of NR1 by PKC decreases its affinity to calmodulin binding, thus preventing the inactivation effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcinurin on the receptor (5).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2904-26A

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

P Type

Pab

Purity

Purified by protein A and peptide affinity chromatography.

Isotype

IgG

References

(1) Malenka, R.C. and Nicoll, R.A. (1999) Science 285, 1870

Additional Info

Detects overexpressed NMDAR1 only when phosphorylated at serine 896. Species Crossreacctivity: Human, mouse and rat.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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