NMDA 2a Receptor (NMDA R2a, N-methyl-D-aspartate)

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kD heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kD, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively. \n \nApplications:\nWestern Blot: 1:1000 \nOptimal dilutions should be determined by each laboratory for each application. \n \nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2905-05B

Size

50ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Serum

References

1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233. \n2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004). \n3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673. \n4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.

Additional Info

Recognizes human, mouse, and rat ~180kD NMDA NR2A. Specific for the 180kD NR2A subunit of the NMDA Receptor in Western Blots of rat hippocampal lysates.