NMDA 2b Receptor (NMDA R2b, N-methyl-D-aspartate, GRIN2B, Glutamate receptor ionotropic N-methyl D-aspartate 2B, HGNC:4586, NR2B, hNR3, N-methyl-D-aspartate receptor subunit 2B)

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.\n\nApplication(s): \nWestern blotting 1:200 (NMDAR2B is present in high concentrations in the hippocampus) Immunoprecipitation: 3ul will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200ug of rat brain using solubilized hippocampal slices. \nImmunohistochemistry: Not suggested\n. \nNote: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.

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SPECIFICATIONS

Catalog Number

N2905-35

Size

50ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Molecular Brain Research (1996) 40:71

Additional Info

NMDAR2B. By Western blot it recognizes a 180kD band in rat brain membranes. Also recognizes a 70kD band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption with the C-terminal fusion protein that was used to generate the antibody. Species Reactivity: Human, rat, mouse and fish.