NMDA Receptor NR2B, phosphorylated (Ser1480) (NMDA R2b, NMDAR2B, N-methyl-D-aspartate, GRIN2B, Glutamate [NMDA] Receptor Subunit epsilon-2, NR2B, N-methyl-D-aspartate Receptor Subunit 2B)

Receptors for NMDA belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Several antagonists and agonists of NMDA receptors (NMDAR) have been identified, including the glutamate analogue homoquinolinic acid, which displays a higher affinity for NR2B-containing NMDAR. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+permeability. Studies have shown that the phosphorylation of the Ser1480 site within the C-terminal PDZ ligand of the NR2B subunit of NMDAR, by casein kinase II (CK2), results in a decrease in neuron NR2B surface expression(1).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2904-35G

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM Hepes, pH 7.5, 0.09% sodium azide, 0.01% BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chung, H.J. et al. (2004) Regulation of the NMDA receptor complex and trafficking by activity-dependent phosphorylation of the NR2B subunit PDZ ligand. J. Neurosci. 24: 10248-10259. 2. Popke, E.J. (2003) TOXOLOGICAL HIGHLIGHT: From anticholinesterase toxicity to Alzheimer

Additional Info

Recognizes the180kD NR2B subunit of the rat NMDA receptor, when phosphorylated at Ser1480. Species sequence homology: mouse, canine, primate, human, bovine, chicken, zebrafish.