NMDAR1 (Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1)

The NMDA receptor (NMDAR) is a specific type of ionotropic glutamate receptor. NMDA (N-methyl D-aspartate) is the name of a selective agonist that binds to NMDA receptors but not to other glutamate receptors. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. NMDAR1 gene is mapped to 9q34.3 and encodes a 938-amino acid protein which showed high evolutionary conservation in structure and physiologic properties. It consists of 21 exons distributed over about 31 kb. Three of the exons that are alternatively spliced in the rat and which produce 8 isoforms in that species were also present in the human sequence. The promoter region contained 2 DNA binding sites for the homeobox proteins 'even-skipped'. The gene is a candidate for the site of the mutation in torsion dystonia.The NMDA receptor is a non-specific cation channel and thus directly contributes to excitatory synaptic transmission by depolarizing the postsynaptic cell. NMDA receptors are modulated by a number of endogenous and exogenous compounds and play a key role in a wide range of physiologic and pathologic processes, such as excitotoxicity.\n\nUniProt Number:\nQ05586\n\nGene ID:\nGRIN1\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144971

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Karp, S. J.; Masu, M.; Eki, T.; Ozawa, K.; Nakanishi, S. : Molecular cloning and chromosomal localization of the key subunit of the human N-methyl-D-aspartate receptor. J. Biol. Chem. 268: 3728-3733, 1993. \n2. Zimmer, M.; Fink, T. M.; Franke, Y.; Lichter, P.; Spiess, J. : Cloning and structure of the gene encoding the human N-methyl-D-aspartate receptor (NMDAR1). Gene 159: 219-223, 1995. \n3. Collins, C.; Duff, C.; Duncan, A. M. V.; Planells-Cases, R.; Sun, W.; Norremolle, A.; Michaelis, E.; Montal, M.; Worton, R.; Hayden, M. R. : Mapping of the human NMDA receptor subunit (NMDAR1) and the proposed NMDA receptor glutamate-binding subunit (NMDARA1) to chromosomes 9q34.3 and chromosome 8, respectively. Genomics 17: 237-239, 1993. \n4. Takano, H.; Onodera, O.; Tanaka, H.; Mori, H.; Sakimura, K.; Hori, T.; Kobayashi, H.; Mishina, M.; Tsuji, S. : Chromosomal localization of the epsilon-1, epsilon-3, and zeta-1 subunit genes of the human NMDA receptor channel. Biochem. Biophys. Res. Commun. 197: 922-926, 1993.

Additional Info

Recognizes human, mouse and rat NMDAR1. No crossreactivity with other proteins.