Nmdar1, NR1 C2 (Glutamate [NMDA] Receptor Subunit zeta-1, N-methyl-D-aspartate Receptor Subunit NR1, NMD-R1, Grin1)

Receptors for NMDA (NMDAR) belong to a group of ionotropic glutamate receptors which play a key role in the mediation of glutamate neurotransmission within the mammalian central nervous system (CNS), including involvement in memory and learning processes. Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage-dependent Mg2+ blockade and high Ca2+ permeability. The NR1 subunit has eight splice variants, arising from either the deletion or insertion of three exon cassettes within the C-terminal (C1, C2) and N-terminal (N1) NR1 domains. These splice variants differ in both their expression and pharmacological properties.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (frozen): 1:1,000-1:2,000\nWestern Blot: 1:1,000\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2904-36

Size

25ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with 50ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ishii, T. et al. (1993) Molecular characterization of the family of the N-Methyl-D-Aspartate receptor subunits. J. Biol. Chem. 268: 2836-2843.2. Ehlers, M.D. et al. (1998) Splice variant-specific interaction of the NMDA receptor subunit NR1 with neuronal intermediate filaments. J. Neurosci. 18: 720-730.

Additional Info

Recognizes the 120kD NR1 subunit of rat NMDA (N-methyl-D-aspartate) receptor, containing the C2 splice variant insert. Does not recognize the NR1 subunit, where the C2 splice variant insert is absent. Species crossreactivity: mouse. Species sequence homology: human.