NMU Receptor 1 (NMUR1)

The neuromedins (Nm) are a family of bioactive peptides best known for their roles in smooth muscle contraction. Nm family of bioactive peptides include: Bombesin-like (NmB, NmC), kassinin-like (NmL and NmK or neurokinins A and B), neurotensin-like (NmN), and neuromedin U (NmU; for its ability to stimulate uterine muscle contraction). Besides its roles in smooth muscle contraction (human ileum, urinary bladder, rat stomach etc), NmU has also been implicated in hypertension, blood flow in intestine, and neurotransmission. Recently two structurally related, orphan G-protein coupled receptors, termed NMUR1 (GPCR66/FM-3/SNORF62) and NMUR2 (TGR-1/FM-4/SNORF72), have been identified as cognate receptors of NmU. NMU receptors display a typical 7 TM domains with extracellular N-terminus and intracellular C-terminus.\nThe human NMUR1 (rat 402 aa; mouse, 405 aa; human 403 aa, chromosome 2; 70-80% interspecies homology) orphan GPCR66 was originally identified by similarity to mouse orthologue FM-3. NMUR1 has moderate sequence homology with neurotensin (NT), and growth hormone secretagogue (GHS, Ghrelin) receptors. NMUR1 is specifically activated by the three peptides (NmU-25, NmU23, and NmU-8) with more or less the same potency. Calcium mobilization assay suggests that NMUR1 is coupled to G-protein of the Gq/11 subfamily. It is highly expressed in rat small intestine and lung. In human, highest expression was found in intestine adipose tissue, with moderate levels of expression in the intestine, lymphocytes, stomach, pancreas, bone marrow, and spleen, and low levels in most other tissues including brain.\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoistochemistry. Other applications not tested.\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL).\nELISA: 1:100,000 using 50-100ng N2911-05 control peptide/well.\nImmunohistochemistry: 2-20ug/ml in formaldehyde fixed tissue\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N2911-07

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Howard AD et al (2000) Nature 406, 70; 2. Fuji R et al (2000) JBC 275, 21068; 3. Raddatz R et al (2000) JBC 275, 32452; 4. Shan L et al (2000) JBC 275, 39482; 5. Hosoya M et al (2000) JBC 275, 29528; 6. Szekeres PG et al (2000) JBC 275, 20247.

Additional Info

Recognizes rat NMU Receptor 1 (NMUR1). Species sequence homology: mouse and human-65% .