Nocodazole (Oncodazole, (METHYL-(5-(2-THIENYLCARBONYL)-1H-BENZIMIDAZOL-2-YL)CARBAMATE, Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate)

Nocodazole, an inhibitor of microtubule formation, was found to greatly inhibit cell division around the mound stage as well as during the vegetative growth phase, when applied to exponentially growing Ax-2 cells. Mitotic arrest of cells with nocodazole can be used to study phosphorylation of microtubule-associated proteins which increase during mitosis, concomitant with increased microtubule dynamics. Nocodazole has been found to dramatically inhibit T cell antigen receptor (TCR) signaling, independently of its ability to depolymerize microtubules. Nocodazole arrests the cell cycle at G2/M phase and prevents phosphorylation of the T cell antigen receptor and inhibits its activity. \n\nMelting Point: \n295-305C\n\nMethod for Determining Identity:\nProton NMR Spectroscopic Analysis\n\nSolvent Solubility (g/100ml):\nDMSO: 1 (with warming)\nFormic Acid: 15.30\nAcetic Acid: 1.39\nLactic Acid: 1.79\nPEG 400: 0.80\nMethanol: 0.026\nNaOH, 0.1N, pH 12.3: 0.015\nEthanol: 0.011\nWater: < 0.0005\nHCl, 0.1N, pH 1.2: 0.007\nCitrate-Phosphate Buffer, pH 8.0: 0.0001\n\nNote: Nocodozole is relatively insoluble. \n\nUsage: Prepare stock solution at 5mg/ml in DMSO. Further dilutions can be made in culture media. Due to insolubility (nocodazole is only slightly soluble in water), no more than 10ug/ml concentration can be used in cell culture systems. Nocodazole is stable in biological media for seven days.\n\nStorage and Stability:\nMay be stored at RT for short-term only. Long-term storage is recommended at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.\n\nToxicity and Hazards:\nDue to the potential danger associated with the use of some of United States Biological biochemicals, all products should be handled by qualified personnel only, trained in laboratory procedures, and familiar with potential hazards. Information is not available on the possible hazards of many experimental compounds. The absence of a warning must not be interpreted as indication of safety. The ultimate responsibility for the proper handling of any and all chemicals lies with the user. All goods sold by United States Biological carry the condition that liability for any injury, loss or damage whatsoever to persons or real property resulting from the handling of the said goods lies with the user. Acceptance of our goods binds the customer to these terms.

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SPECIFICATIONS

Catalog Number

N3000

Size

20mg

Form

Supplied as a light tan to brown powder

Purity

~98% (HPLC)

References

US Biological application reference: Jones, M. H. et al., (1999) Mol. Biol. Cell 10: 2377-2391. 1. Rennison, M.E., et al., (1992) J. Cell Sci. 102:239. 2. Fleischman, L.F., et al., (1993) Oncogene 8:771. 3. Amon, I., et al., (1994) 77:1037-1050. 4. Araki, T., Maeda, Y., (1998) Zoolog. Sci. 15(1):77-84. 5. llenberger, S., et al., (1998) Mol. Biol. Cell. 9(6):1495-1512. 6. Huby, R.D., et al.,(1998) J. Biol. Chem.15,273(20):12024-12031.

Alternative Names

METHYL-(5-(2-THIENYLCARBONYL)-1H-BENZIMIDAZOL-2-YL)CARBAMATE; Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate