NOD2 (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)

Innate immunity is present in all animals and is the common mode of defense against microorganisms. It detects microorganisms by specific proteins called pattern-recognition molecules (PRMs). There is a limited set of PRMs in each animal genome, and it has been postulated that the PRMs were evolutionarily selected to detect conserved components or motifs of microorganisms called pathogen-associated molecular patterns (PAMPs). PAMPs are found in a wide range of microorganisms and recognition of PAMPs by PRMs activates inflammatory signaling pathways, thereby stimulating an immune response. NOD (nucleotide-binding oligomerization domain) proteins are a family of cytosolic proteins which have been implicated in innate recognition of bacteria, the induction of inflammatory responses, and the regulation of caspase activation and apoptosis. NOD2:CARD15 (caspase-recruitment domain 15) is a PRM that recognizes specific peptidoglycan (PGN) components of bacterial cell walls. NOD2 is expressed in cells that are exposed to PGN under physiological conditions including antigen-presenting cells (APCs) such as macrophages and dendritic cells, and epithelial cells. NOD2 is a for thought to play a role in the pathogenesis of human gastrointestinal disease. Polymorphisms and mutations in NOD2 are associated with susceptibility to Crohn's disease, a chronic inflammatory bowel disease. Additionally, polymorphisms in NOD2 have also been found to be associated with Graft-versus-Host Disease (GVHD). This association of NOD2 gene polymorphisms with gastrointestinal disease and GVHD suggests that NOD2 gene polymorphisms play a role in pro-inflammatory diseases. NOD2 is a 1040aa protein.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000\nImmunoprecipitation: 1:50-1:200\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N3006-14G

Size

50ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Werts C, SE Girardin, and DJ Philpott. 2006. TIR, CARD and PYRIN: three domains for an antimicrobial triad. Cell Death Diff. 13:798-815. 2. Inohara N and G Nunez. 2003. NODS: Intracellular proteins involved in inflammation and apoptosis. Nature Rev/Immunology. 371-382. 3. Holler E, G Rogler, H Herfarth, et al. 2004. Both donor and recipient NOD2/CARD15 mutations associate with transplant-related mortality and GvHD following allogeneic stem cell transplantation. Blood 104:889-894, 4. Strober W, PJ Murray, A Kitani and T Watanabe. 2006. Signalling pathways and molecular interactions of NOD1 and NOD2. Nature Rev/Immunology. 6:9-20. 5. Leung E, J Hong, A Fraser, and GW Krissansen. 2006. Splicing of NOD2 (CARD15) RNA transcripts. Molecular Immunology. doi:10.1016/j.molimm.2006.03.009.

Additional Info

Recognizes human NOD2 alternatively spliced and mutated forms which contain the peptide immunogen sequence including NOD2 short.