Noelin-1, Recombinant, Mouse (Neuronal Olfactomedin-related Endoplasmic Reticulum-localized-1, Olfactomedin-1, Pancortin)

Noelin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as olfactomedin-1 or pancortin, is a 75kD\\ secreted extracellular matrix glycoprotein expressed in the brain.1-3 Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1-4) that are combinations of a common central region with alternate N and C termini.3, 4 Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain.4 In the species tested, these isoforms appear to oppose actions of the longer isoforms.5 All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region.2 The C-terminus of Noelin-1 (mouse aa 482-485) contains a putative endoplasmic reticulum retention sequence that has not been included in the protein.6 Mouse Noelin-1 (aa 17-481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species.1 In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells.7 Overproduction of Noelin-1 prolongs neural crest cell formation.7 Noelin-1 is also expressed in mouse neural crest, but not frog.1-3 All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia.1-3\n\nA DNA sequence encoding mouse Noelin-1 (Met 1-Arg 481; Accession # NP_062371) was fused with a six-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.\n\nMolecular Mass:\nCalculated molecular mass of ~ 55.7kD. As a result of glycosylation, the recombinant mouse Noelin-1 migrates as an ~ 80-90kD protein in SDS-PAGE under reducing conditions.\n\nActivity: \nMeasured by its ability to induce epithelial to mesenchymal transformation of MDCKII cells. Treatment of MDCKII cells with 200ng/ml of recombinant mouse Noelin-1 for 3 days is sufficient to induce the transformation of MDCKII cells.\n\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N3040

Size

50ug

Form

Supplied as a lyophilized powder from PBS, pH 6.5. Reconstitute with sterile PBS to a concentration of (same/more than)100ug/ml.

Purity

(same/more than) 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.\nEndotoxin: (same/less than) 1EU/1ug.

References

1. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121. 2. Ando, K. et al. (2005) Neuroscience 133:947. 3. Nagano, T. et al. (1998) Brain Res. Mol. Brain Res. 53:13. 4. Danielson, P.E. et al. (1994) J. Neurosci. Res. 38:468. 5. Moreno, T.A. & M. Bronner-Fraser (2005) Dev. Biol. 288:434. 6. Moreno, T.A. & M. Bronner-Fraser (2001) Dev. Biol. 240:340. 7. Barembaum, M. et al. (2000) Nat. Cell Biol. 2:219.