Nogo 66 Receptor (Ngr)

Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrow after injury. The CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses. Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized. It may help block the regeneration of the CNS. Nogo is the 4th member of reticulon (Rtn) family. There are three alternative isoforms of Nogo, designated Nogo-A (full length), an intermediate form Nogo-B (373aa; ~37 K, lacks 186-1004aa within the extracellular domain), and a shorter form Nogo-C (199aa; ~25 K, similar to rat vp20 and foocen-s; lacks 186-1004aa but which has a smaller, alternative N-terminal domain). A 66aa hydrophilic region of Nogo (Nogo-66), located between the two TM domains, has the most inhibitory properties of Nogo. In contrast to Nogo, Rtn 1, -2 and 3 do not inhibit axonal regeneration. This 66aa region also has the least similarity to Rtn proteins. The corresponding Rtn-P4 peptide sequence has no activity. A brain specific leucine-rich-repeat protein with high affinity for soluble Nogo-66, termed Nogo receptor (Ngr) has been cloned and characterized. Cleavage of Ngr from the axonal surface renders neurons insensitive to Nogo-66. Ngr expression is sufficient to impart Nogo-66 axonal inhibition to unresponsive neurons. Ngr protein (mouse, rat, monkey, and human 473aa; chromosome 22q11) contains a signal sequence followed by eight LRR domains, an LRR cysteine-rich CT-flanking domain, and unique GPI anchorage site. Human and mouse Ngr proteins are ~89% identical. Ngr is expressed in brain and lower levels are also detected in hear and kidney but not in other peripheral tissues. Ngr has been localized to axons.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN2116-95C: Nogo 66 Receptor, Human (Ngr)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2116-95A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 005% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Fournier, A.E., et al., Nature 409: 341-346 (2001). 2. Chen, M.S., et al., Nature 403: 434-439 (2000). 3. GrandPre, T., et al., Nature 403: 439-444 (2000). 4. Goldberg, J.L., Barres, B.A., Nature 403: 369-370 (2000). 5. Prinjha, R., et al., Nature 403: 383-384 (2000). 6. Tessier-Lavigne, M., Goodman, C.S., Science 287: 813-814 (2000). 7. Nagase, T., et al., DNA Res. 5: 355-364 (1998).

Additional Info

Recognizes Nogo 66 Receptor. No significant sequence homology is detected with Rtn family members. Species sequence homology: Human, mouse, rat and monkey Ngr: 100%.