NogoA, NT (Neurite Outgrowth Inhibitor A)

NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients. Despite its predicted molecular weight, NogoA typically migrates at 180kD in an SDS-PAGE. At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMouse brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2116-45D

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Yan R, et al (2006) Cell. Mol. Life Sci. 63:877-889. 2. Chen MS, et al (2000) Nature. 403:434-9. 3. Schweigreiter R, et al (2006) J. Neurotrauma. 3- 4:384-96. 4. Dupuis L, et al (2002) Neurobiol. Dis. 10:358-65. 5. Reinl M, et al (2003) J. Neuroimmunol. 145:139-47.

Additional Info

Recognizes human NogoA. Species Crossreactivity: mouse and rat.