NONO, CT (Non-POU Domain-containing Octamer-binding Protein, NonO Protein, 54kD Nuclear RNA- and DNA-binding Protein, 55kD Nuclear Protein, DNA-binding p52/p100 Complex, 52kD Subunit, NMT55, p54(nrb), p54nrb, NRB54)

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54kD nuclear RNA- and DNA-binding protein, p54 (nrb), 55kD nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.3ug/ml\nELISA: 1:16,000\nImmunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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