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Nop2p

Cat no: N2984-10A

Nop2p

Nop2p is the yeast homologue of the human p120 nucleolar 'proliferation' antigen. The Nop2p protein is localized in the nucleolus. \n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications not tested. \n\nRecommended Dilution:\nWestern Blots of yeast protein samples: 1:5000 (cell lysates) to 1:25,000 (nuclear fraction), followed by chemiluminescent detection (ECL). For other (non-ECL) Western detection methods: 1:500-1:2500. \nImmunofluorescence on yeast cells: 1:250-1:2500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2984-10A

Size

100ug

Applications

IF, WB

Hosts

Mouse

Reactivities

Yeast/Fun

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes yeast nucleolar marker, which is a putative SAM-dependent methyl transferase.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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