Noradrenaline (arterenol) (Biotin)

Applications: \nSuitable for use in ELISA, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:1000-1:2500\nImmunocytochemistry: 1:1000-1:2500\nOptimal dilutions to be determined by the researcher.\n\nAb:Biotin Label Ratio:\n1:4.7\n\nSpecificity:\nUsing a conjugate Noradrenaline-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds (1).\n\nCompound Crossreactivity ratio (a):\nNoradrenaline-G-BSA 1\nOctopamine-G-BSA 1:31\nDopamine-G-BSA 1:70\nAdrenaline-G-BSA 1:177\nL-DOPA-G-BSA > 1:5000\nTyramine-G-BSA > 1:5000\nNoradrenaline > 1:5000\n\n(a): Noradrenaline-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement.\nG = Glutaraldehyde, BSA = Bovine Serum Albumin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N3900-01

Size

50ug

Applications

ELISA, ICC, IHC

Hosts

Rabbit

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, before the addition of 40% glycerol. No preservatives added. Labeled with Biotin.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG

References

o CAFFE A.R., VAN LEEUWEN F.W., BUIJS R.M., DE VRIES G.J. and GEFFARD M. Coexistence of vasopressin, neurophysin and noradrenaline immunoreactivity in medium-sized cells of the locus coeruleus and subcoeruleus in the rat. Brain Res., (1985) 338, 160-164.\no GEFFARD M., PATEL S., DULLUC J. and ROCK A.M. Specific detection of noradrenaline in the rat brain by using antibodies. Brain Res., (1986) 363, 395-400.\no Mc RAE-DEGUEURCE A. and GEFFARD M. One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain. Brain Res., (1986) 376, 217-219.\no MONS N. and GEFFARD M. Specific antisera against the catecholamines : L-3,4- dihydroxyphenylalanine, dopamine, noradrenaline and octopamine tested by and enzyme-linked immunosorbent assay. J. Neurochem., (1987) 48, 1826-1833.\no DECAVEL C., GEFFARD M. and CALAS A. Comparative study of dopamine and noradrenaline immunoreactive terminals in the paraventricular and supraoptic nuclei of the rat. Neurosc. Letters, (1987) 77, 149-154.\no SEGUELA P., WATKINS K.C., GEFFARD M. and DESCARRIES L. Noradrenaline axon terminals in adult rat neocortex : an immunocytochemical analysis in serial thin sections. Neuroscience, (1990), 35, 249-264.\no FRITSCHY J.M., GEFFARD M. and GRZANNA R. The response of noradrenergic axons to systematically administered DSP-4 in the rat : an immunohistochemical study using antibodies to noradrenaline and dopamine b-hydroxylase. J. of Chemical Neuroanatomy, (1990), 3, 309-315.\no MAEDA T., KOJIMA Y., ARAI R., FUJIMIYA M., KIMURA H., KITAHAMA K. and GEFFARD M. Monoaminergic interaction in the central nervous system : a morphological analysis in the locus coeruleus of the rat. Comp. Biochem. Physiol., (1991), 98c, 193-202.\no DELAGRANGE P., CONRATH M., GEFFARD M., TADJER D., BOUYER J.J. and ROUGEUL A. Noradrenaline-like terminals in the cat nucleus ventralis posterior of the thalamus. Brain Res. Bull., (1991), 26, 533-538.\no RAJAOFETRA N., POULAT N., MARLIER L., SANDILLON F., DRIAN M.J., KONIG N., FAMOSE F., VERSCHUERE B., GOUY D., GEFFARD M. and PRIVAT A., Transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (Macaca fascicularis). Brain Res. (1992) 572, 329-334.\no RIDET J.L., RAJAOFETRA N., GEFFARD M. and PRIVAT A., Reconstruction tridimensionnelle des projections noradr

Additional Info

Recognizes Noradrenaline. See additional crossreactivity data.