Norepinephrine Transporter (NET)

Noradrenaline transporter or epinephrine transporter (NET or NTT1 or NAT1 or SLC6A2) gene (chromosome 16) encodes a protein of 617aa (mouse 617aa, ~94% identity), with 12-13 transmembrane domains, and cytoplasmic N and C-termini. Expression of the cDNA clone in transfected HeLa cells indicated that noradrenaline transport activity is sodium-dependent and sensitive to selective noradrenaline transport inhibitors. NET mRNA has been localized to the brain stem and adrenal. The predicted protein sequence demonstrated significant amino acid identity with the Na(+)/gamma-aminobutyric acid transporter, thus identifying a new gene family for neurotransmitter transport proteins. Alternative splicing of NET mRNA produces two transporter isoforms, rNETa and rNETb, which differ at their COOH termini. The rNETa isoform reveals a COOH terminus homologous to human NET and transports norepinephrine. In contrast, rNETb revealed no detectable transport function, but reduced functional expression of rNETa when both isoforms were expressed in the same cell. Defects in NET are a cause of orthostatic intolerance (OI), a syndrome characterized by lightheadedness, fatigue, altered mentation and syncore. It is associated with postural tachycardia.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using ECL.\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: N3950-06\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N3950-05A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide with 40% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Bruss, et al., 1995, J.B.C. 270: 9,197-9,201. 2. Pacholczyk, et al., 1991, Nature 350: 350-354. 3. Melikian, et al., 1994 J.B.C. 269: 12,290-12,297. 4. Frita, J.D., et al., 1998, J. Neurochem. 70: 2241-2251. 5. Poezgen, P., et al., 1995, B.B.R.C. 215: 1,145-1,150. 6. Kotayama, S., et al., 1999, J.B.C. 274: 10,731-10,736.

Additional Info

Recognizes rat NET. Species sequence homology: mouse-100%, human, monkey and bovine-90%, chicken-85%.