Normetanephrine, Human, BioAssay(TM) ELISA Kit

Sample Type: \nUrine\n\nIntended Use:\nEnzyme immunoassay for the manual and automated quantitative determination of normetanephrine in human urine.\n\nTest Principle:\nThe assay procedure follows the basic principle of competitive ELISA whereby there is competition between a biotinylated and a non-biotinylated antigen for a fixed number of antibody binding sites. The amount of biotinylated antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the free biotinylated antigen is removed by a washing step and the antibody bound biotinylated antigen is determined by use of anti-biotin alkaline phosphatase as marker and p-nitrophenyl phosphate as substrate. Quantification of unknowns is achieved by comparing the enzymatic activity of unknowns with a response curve prepared by using known standards.\n\nKit Components:\nAssay Buffer, Concentrate (10X), 1x50ml\nAcylation Reagent, 1x2.25ml \nIndicator Buffer, 1x10ml\nHydrolyzation Tubes, 2x50each\nHCl Solution, 0.1M HCl, 1x20ml \nMicrotiter plate, Coated with anti-rabbit IgG (goat, polyclonal), 1x96 wells\nStandard A-G (7x0.35ml)\nStandard, 0ug/L, 1x350ul\nStandard, 77ug/L, 1x350ul\nStandard, 192ug/L, 1x350ul\nStandard, 480ug/L, 1x350ul\nStandard, 1200ug/L, 1x350ul\nStandard, 3000ug/L, 1x350ul\nStandard, 7500ug/L, 1x350ul\nControl 1, 1x0.5ml\nControl 2, 1x0.5ml\nNormetanephrine Biotin, 3x1vial\nNormetanephrine Antiserum, 1x7ml\nEnzyme Conjugate, Concentrate (100X), 1x250 ul\nSubstrate Tablets, Contains: p-nitrophenyl phosphate (PNPP), 9x1each\nSubstrate Buffer, 1x27ml \nStop Solution, 1x15ml \nWash Buffer, Concentrate (10X), 1x50ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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