NOS III (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitters, NO is not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.\nNO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principal group is inducible, Ca2+/calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%. Human, rat, mouse and bovine eNOS/NOS-3 are ~1202-1205aa proteins.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: Coat plates with control peptide (Cat# N5350-28A) at 1ug/ml.\nWestern Blot: 1-10ug/ml; Recognizes 135-140kD protein.\nImmunocytochemistry/Immunohistochemistry: 5-10ug/ml. Useful on tissue sections fixed with 3.5% paraformaldehyde.

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SPECIFICATIONS

Catalog Number

N5350-28B

Size

50ug

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Marsden, P.A., FEBS Lett. 307: 287-293 (1992). 2. Janssens, S.P., J. Biol. Chem. 267: 14,519-14,522 (1992). 3. Michel, T. & Lamas, S., J. Card. Pharmacol. 20: S45-S49 (1992). 4. El-Omar, M.M., J. Heart Failure 5: 229-239 (2003). 5. Ulbrich, S.E., J. Endocrinol. 188: 205-213 (2006). 6. Dorenkamp, M., Eur. J. Pharmacol. 520: 179-187 (2005). 7. Bianca, D.V., J. Pharmacol. Exp. Ther. 316: 703-708 (2006).

Additional Info

Recognizes the 135-140kD protein human endothelial nitric oxide synthase (eNOS, NOS III). Species Sequence Homology: mouse, bovine - 100% (15/15aa); rat - 93% (14/15aa); porcine - 86% (13/15aa). No significant sequence homology is seen with NOS-1/NOS-2 or other proteins.