NOS III, Mouse, Control Peptide (Nitric Oxide Synthase 3, Nitric Oxide Synthase 3 Endothelial Cell, NOS Type III, NOS3, Endothelial Nitric Oxide Synthase 3, Endothelial NOS, eNOS, ECNOS, EC-NOS, Constitutive NOS, cNOS, Nitric Oxide Synthase Endothelial)

Control Peptide for N5350-29 (antiserum). Synthetic peptide consisting of 20aa sequence near the C-terminus of mouse eNOS. No significant sequence homology is seen with NOS-1/NOS-2 or other proteins. Species Sequence Homology: Rat, human, bovine, canine: Conserved.\n\nNitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~50%. Human, rat, mouse and bovine eNOS/NOS-3 are ~1202-1205aa proteins.\n\nApplications: \nSuitable for use in ELISA, and antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested.\n\nRecommended Dilution:\nELISA: Caot wells at 1ug/ml.\nAntibody Blocking: 5-10ug per 1ul N5350-29\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5350-20E

Size

50ug

Applications

ELISA

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. Marsden, P.A., Febs Lett. 307: 287-293 (1992). 2. Jaanssens, S.P., J. Biol. Chem. 267: 14519-14522 (1992). 3. Michel, T., Lamas, S., J. Card. Pharmacol. 20: S45-S49 (1992).