NOS III, NT (Endothelial Nitric Oxide Synthase, eNOS, NOS 3)

Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).\n\neNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.\n\nApplications: \nSuitable for use in Western Blot and immunocytochemical . Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:800\nImmunocytochemistry: 1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

E3218-01A

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Reconstitute with 0.1ml PBS, 10mg/ml BSA.

P Type

Pab

Purity

Serum

Additional Info

Recognizes human eNOS. Cross-reactivity: eNOS (2-11 Cys12): 100%; recombinant eNOS (NOS type III): 100%; eNOS (cap-2-11Cys12): 50%; recombinant myristylated eNOS (myristylated NOS type III): 50%; recombinant hnNOS (NOS type I): 0%; recombinant hiNOS (NOS type II): 0%.