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NOS Primers, Hu neuronal amplimer 471bp

Cat no: N2991-10

NOS Primers, Hu neuronal amplimer 471bp

Contents:\nPCR primers for use in the detection and analysis of human mRNA transcript. This PCR-Amplimer set amplifies a specific gene transcript of size 471bp. The amplimer set includes 5' and 3' primers in separate tubes compatible with regular PCR reagents. Each set (each primer in a 50ul volume) is sufficient for at least 100 reactions.\n\nPrimer A: Sense Primer\nPrimer B: Antisense Primer\n\nStorage and Stability:\nStore at -20 degrees C in a constant-temperature freezer.\n\nOptimal PCR Conditions:\nNumber of cycles: 30\nDenaturation temperature: 96 degrees C for 35 secs.\nAnnealing temperature: 56 degrees C for 2 min.\nExtension temperature: 72 degrees C for 2 min. in the presence of 2mM MgCl2.

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SPECIFICATIONS

Catalog Number

N2991-10

Size

100Tests

Form

Supplied in sterile water.

References

Garvey, E.P., Tuttle, J.V., Covington, K., Merrill, B.M., Wood, E.R., Baylis S.A., and Charles, I.G., Arch. Biochem. Biophys.,

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