Notch 2, Cleaved, NT (Notch2, Notch-2, Aicardi-Goutieres Syndrome 2, AGS2, Drosophila Notch Homolog 2, hN2, N2, Neurogenic Locus Notch Homolog Protein 2 Precursor, Notch (Drosophila) Homolog 2, Notch Homolog 2 (Drosophila), Notch Homolog 2)

Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:30000-1:90000\nWestern Blot: 1:500-1:1500\nImmunohistochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nNIH/3T3 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-15L

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 0.15M sodium chloride.

P Type

Pab

Purity

Serum

Isotype

IgG

Additional Info

Recognizes human Notch 2, cleaved N-terminal. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form. Species Crossreactivity: mouse. Species sequence homology: rat.