Notch 2 (Notch2, Notch-2, Aicardi-Goutieres Syndrome 2, AGS2, Drosophila Notch Homolog 2, hN2, N2, Neurogenic Locus Notch Homolog Protein 2 Precursor, Notch (Drosophila) Homolog 2, Notch Homolog 2 (Drosophila), Notch Homolog 2)

Notch-2 (also Motch-2) is a 280-300kD member of the notch family of transmembrane (TM) proteins. It is found on multiple cell types, and exbitits multiple functions. On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation. Mature mouse Notch-2 is a 2445aa type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200kD extracellular domain (ECD) (aa26-1606) possibly covalently-linked to a 100-110kD membrane bound C-terminal segment (aa1607-2470). Upon binding to Delta1, Jagged1, or Jagged2, the 110kD segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor. There are three potential splice variants. One shows a 32aa substitution for (aa 293-2470), a second contains a 13aa substitution for (aa1-1954), and a third possesses a five aa substitution for (aa224-227) coupled to an 11aa substitution for (aa1704-1713). Over (aa26-528), mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1ug/ml\nWestern Blot: 2ug/ml\nOptimal dilutions to be determined by the researcher\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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